Data Publication

Database of in-situ Raman spectra from Na2H2ATP solutions at 80,100 and 120 °C and up to 1666 MPa for determination of the rate constant of the ATP hydrolysis

GFZ Data Services

(2023)

Descriptions

In biochemical systems, enzymes catalyze the endergonic phosphorylation of adenosine diphos-phate (ADP) to adenosine triphosphate (ATP) by different pathways, e.g., oxidative phosphoryla-tion catalyzed by membrane bound ATP synthase or substrate-level phosphorylation. The stored energy is released by the enzymatically controlled exergonic hydrolysis of ATP to power other vital endergonic reactions; therefore, ATP is widely known as the universal energy currency. Rapid abiotic ATP hydrolysis kinetics thus means higher maintenance energy costs for cells, and it has been suggested that this is an important factor in setting the limits to the functioning of living organisms (Bains et al. 2015). In order to evaluate the running conditions of the in-situ procedure by Moeller et al. (2022) using Raman spectroscopy opened up an efficient way of obtaining further insights to the effects of P-T- ionic composition on the kinetics of ATP-ADP hy-drolysis. Raman spectroscopy can be combined with a hydrothermal diamond anvil cell, which provides an isochoric system for measurements up to pressures of 2000 MPa. Another system for in-situ Raman spectroscopy at elevated pressures and temperatures is based on an autoclave fitted with optical high-pressure windows, as shown by Louvel et al. (2015) and works up to 200 MPa. In this system, pressure and temperature can be controlled independently, so that isobaric temperature series are possible. This data publication compromises all Raman spectra measured in-situ of Na2H2ATP solutions at 80, 100 and 120 °C and up to 1666 MPa to determine the rate constants of the hydrolysis of adenosine triphosphate (ATP) to adenosine diphosphate (ADP) at 48 different P-T conditions. Furthermore, an assignment of peaks in the fitted range, the initial fit parameters and the fit-results are provided. Besides the kinetic data, the pH of the ATP solutions was calculated at experimental temperature and pressure conditions.

Keywords

Originally assigned keywords

ATP

adenosine triphosphate

ADP

adenosine diphosphate

AMP

adenosine monophosphate

In-situ Raman spectroscopy

Autoclave

Hydrothermal diamond anvil cell

HDAC

Hydrolysis

Kinetics

biosphere > biological process > animal life > metabolism > metabolite

chemical process > chemical reaction > reaction kinetics

EARTH SCIENCE > OCEANS > MARINE VOLCANISM > HYDROTHERMAL VENTS

MSL enriched keywords

Technique

chemical and mineralogical analysis

Raman spectrometry - chemistry and mineralogy

crystal structure analysis

Raman spectrometry - crystal lattice

minerals

chemical elements

carbon

diamond

Apparatus

deformation testing

compression testing

diamond anvil cell

measured property

pH

MSL enriched sub domains i

microscopy and tomography

rock and melt physics

geochemistry

Source

https://dataservices.gfz-potsdam.de/panmetaworks/showshort.php?id=6174101d-6828-11ee-967a-4ffbfe06208e

Source publisher

GFZ Data Services

DOI

10.5880/fidgeo.2023.031

Creators

Moeller, Christoph

Institute of Geosciences, University of Potsdam, Potsdam, Germany

ORCID:

https://orcid.org/0000-0001-7824-8004

Schmidt, Christian

GFZ German Research Centre for Geosciences, Potsdam, Germany

Testemale, Denis

Néel Institute, Univ. Grenoble Alpes, Grenoble, France

Guyot, François

IMPMC Muséum National d'Histoire Naturelle, Paris, France

ORCID:

https://orcid.org/0000-0003-4622-2218

Kokh, Maria

Institut für Geowissenschaften, Potsdam, Germany; Institut für Mineralogie, Münster, Germany

ORCID:

https://orcid.org/0000-0002-0855-015X

Wilke, Max

Institute of Geosciences, University of Potsdam, Potsdam, Germany

ORCID:

https://orcid.org/0000-0002-1890-3940

Contributors

Moeller, Christoph

ContactPerson

Institute of Geosciences, University of Potsdam, Potsdam, Germany

ORCID:

https://orcid.org/0000-0001-7824-8004

Moeller, Christoph

DataCollector

Institute of Geosciences, University of Potsdam, Potsdam, Germany

ORCID:

https://orcid.org/0000-0001-7824-8004

Schmidt, Christian

DataCollector

GFZ German Research Centre for Geosciences, Potsdam, Germany

Testemale, Denis

DataCollector

Néel Institute, Univ. Grenoble Alpes, Grenoble, France

Guyot, François

Researcher

IMPMC Muséum National d'Histoire Naturelle, Paris, France

ORCID:

https://orcid.org/0000-0003-4622-2218

Kokh, Maria

DataCollector

Institut für Geowissenschaften, Potsdam, Germany; Institut für Mineralogie, Münster, Germany

ORCID:

https://orcid.org/0000-0002-0855-015X

Wilke, Max

Supervisor

Institute of Geosciences, University of Potsdam, Potsdam, Germany

ORCID:

https://orcid.org/0000-0002-1890-3940

Hydrothermal Diamond-Anvil Cell Laboratory

HostingInstitution

GFZ German Research Centre for Geosciences, Potsdam, Germany

Laboratory Of Fundamental Research In Condensed Matter Physics

HostingInstitution

Néel Institute, Grenoble, France

Moeller, Christoph

ContactPerson

Institute of Geosciences, University of Potsdam, Potsdam, Germany

Moeller, Christoph

ContactPerson

Institute of Geosciences, Potsdam, Germany

References

10.1016/j.gca.2024.06.017

10.3390/life5021054

10.1007/bf00998341

10.1088/1742-6596/121/12/122003

10.1039/c0dt00417k

10.1002/jrs.1250190807

10.1021/bi00783a004

10.1016/0301-4622(94)00134-6

10.1016/s0026-2692(01)00087-8

10.1016/j.molliq.2014.09.032

10.1039/f29898501963

10.1016/0008-6215(84)85398-7

10.1016/j.bpc.2022.106878

10.1021/j100470a011

10.1016/s0006-3495(69)86389-7

10.1180/emu-notes.12.7

10.2138/am-2000-11-1216

10.1063/1.1884188

Citation

Moeller, C., Schmidt, C., Testemale, D., Guyot, F., Kokh, M., & Wilke, M. (2023). Database of in-situ Raman spectra from Na2H2ATP solutions at 80,100 and 120 °C and up to 1666 MPa for determination of the rate constant of the ATP hydrolysis [Data set]. GFZ Data Services. https://doi.org/10.5880/FIDGEO.2023.031

Dates

Issued:

2023

Rights

Creative Commons Attribution 4.0 International