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Metadata Files

Data Publication

Data presented in the paper ‘High prevalences of disseminated neoplasia in the Baltic tellin Limecola balthica in the Wadden Sea’

Dairain, A. (Annabelle) | Engelsma, M.Y. (Marc) | Drent, J. (Jan) | Dekker, R. (Rob) | Thieltges, D.W. (David)

4TU.Centre for Research Data

(2020)

Descriptions

This work aimed at investigating the presence of disseminated neoplasia in the Baltic clam Limecola balthica. We conducted a field survey in the Dutch Wadden Sea (10 sampling sites) to determine the prevalence of the disease in Baltic tellins in the Wadden Sea and to quantify its severity via histology. Briefly, bivalves were sampled by brushing away the first 10 cm of the sediment surface. Organisms were then collected by hand and keep in the Davidson’s fixative on back in the lab. Tissues of samples were then collected and embedded in paraffin blocks. Histological sections of 5 µm thickness were stained with haematoxylin and eosin (H&E), and the slides examined for the presence of disseminated neoplasia using light microscopy. If neoplastic cells were present, we determined the disease severity according to Christensen et al. (1974) and Pekkarinen (1993) as follows: stage 1, local neoplastic cells noticed in the gills; stage 2, gills totally invaded with neoplastic cells; stage 3, spots of neoplastic cells in other tissues than gills, and stage 4, widespread neoplastic cells. For each sampling site, we calculated the prevalence of the disease and the prevalence of each stage of the disease. The data set is an excel file, with different columns, specifying where and when bivalves were sampled, the type of environment (sandy mud, muddy sand or sand), the density of the bivalve at each location, and the stage of the disease (O to 4).

Keywords


Originally assigned keywords
Zoology
FOS: Biological sciences
Ecology
Veterinary Sciences
FOS: Veterinary sciences
Bivalvia
Marine Ecology
Mollusca
disease
Time: 2014-02/2019-03

MSL enriched keywords
unconsolidated sediment
clastic sediment
mud
sand
analogue modelling material
viscous modelling material
natural viscous material
paraffin
synthetic viscous material
paraffin
Apparatus
optical microscopy

MSL enriched sub domains i

microscopy and tomography

Source

https://data.4tu.nl/articles/_/12703181/1

Source publisher

4TU.Centre for Research Data


DOI

10.4121/uuid:6d4e69a4-d373-42c8-999e-dfa5d3ea340b


Creators

Dairain, A. (Annabelle)

https://orcid.org/:

0000-0003-4513-155X

Engelsma, M.Y. (Marc)

https://orcid.org/:

0000-0001-7683-8596

Drent, J. (Jan)

https://orcid.org/:

0000-0003-1277-5957

Dekker, R. (Rob)

Thieltges, D.W. (David)

https://orcid.org/:

0000-0003-0602-0101


Contributors

NIOZ Royal Netherlands Institute For Sea Research, Department Of Coastal Systems, And Utrecht University

Other

Wageningen Bioveterinary Research, Part Of Wageningen University And Research

Other


References

https://doi.org/10.3354/dao03448


Citation

Dairain, A. (A., Engelsma, M. Y. (M., Drent, J. (J., Dekker, R. (R., & Thieltges, D. W. (D. (2020). Data presented in the paper ‘High prevalences of disseminated neoplasia in the Baltic tellin Limecola balthica in the Wadden Sea’ (Version 1) [Data set]. 4TU.Centre for Research Data. https://doi.org/10.4121/UUID:6D4E69A4-D373-42C8-999E-DFA5D3EA340B


Dates

Issued:

2020-01-16


Language

en


Rights

Creative Commons Zero v1.0 Universal


Datacite version

1


Geo location(s)

Dutch Wadden Sea (tidal flats)