Data Publication

VERDI [Dataset]

CSIC - Instituto de Ciencias del Mar (ICM)

(2022)

Descriptions

The presence of phytoplankton parasites along the water column was explored at the Long Term Ecological Station MareChiara (LTER-MC) in the Gulf of Naples (Mediterranean Sea) in October 2019. Microscopy analyses showed diatoms dominating the phytoplankton community in the upper layers (0-20 m). Metabarcoding data from the water column showed the presence of Chytridiomycota predominantly in the upper layers coinciding with the vertical distribution of diatoms. Laboratory incubations of natural samples enriched with different diatom cultures confirmed parasitic interactions of some of those chytrids – including members of Kappamyces – with diatom taxa. The temporal dynamics of diatoms and chytrids was also explored in a three-year metabarcoding time-series (2011-2013) from surface waters of the study area and in sediment samples. Chytrids were recurrently present at low relative abundances, and some taxa found to infect diatoms in the incubation experiments were also identified in the ASV time-series. However, co-occurrence analyses did not show any clear or recurrent pairing patterns for chytrid and diatom taxa along the three years. The chytrid community in the sediments showed a clearly different species composition compared to the recorded in the water column samples, with higher diversity and relative abundance. The combination of observations, incubations and metabarcoding confirmed that parasites are a common component of marine protist communities at LTER-MC. Host-parasite interactions must be determined and quantified to understand their role and the impact they have on phytoplankton dynamics

File1: VERDI_samples_parameters.xlsx - Physico-chemical variables obtained from CTD profile - Inorganic nutrients concentrations - Chlorophyll-a concentrations - Organic carbon and nitrogen concentrations - Phytoplankton abundances - Detections of chytrids File 2: VERDI_asv_table.tbl: ASV abundances from natural samples and incubations File 3: VERDI_tax_table.tbl: Taxonomic assignments of ASVs File 4: VERDI_asv_seqs.fa: Sequences of ASVs File 5: VERDI_incubations_images.zip - Compilation of images taken during incubations with diatoms

- Physico-chemical variables obtained from CTD profile - Inorganic nutrients concentrations - Chlorophyll-a concentrations - Organic carbon and nitrogen concentrations - Phytoplankton abundances - Detections of chytrids - Metabarcoding ASV abundances from natural samples and incubations - Metabarcoding Taxonomic assignments of ASVs - Metabarcoding Sequences of ASVs - Compilation of images taken during incubations with diatoms

Keywords

Originally assigned keywords

Parasites

Chytrids

Diatoms

LTER

Metabarcoding

MSL enriched keywords

minerals

chemical elements

carbon

measured property

carbon (C)

organic carbon

nitrogen

MSL enriched sub domains i

geochemistry

Source

https://digital.csic.es/handle/10261/279412

Source publisher

CSIC - Instituto de Ciencias del Mar (ICM)

DOI

10.20350/digitalcsic/14744

Creators

Reñé, Albert

Timoneda Solé, Natàlia

Sarno, Diana

Zingone, Adriana

Margiotta, Francesca

Passarelli, Augusto

Gallia, Roberto

Tramontano, Ferdinando

Montresor, Marina

Garcés, Esther

Contributors

Digital.CSIC

DataManager

Digital.CSIC

HostingInstitution

Citation

Reñé, A., Timoneda Solé, N., Sarno, D., Zingone, A., Margiotta, F., Passarelli, A., Gallia, R., Tramontano, F., Montresor, M., & Garcés, E. (2022). VERDI [Dataset] [Data set]. CSIC - Instituto de Ciencias del Mar (ICM). https://doi.org/10.20350/DIGITALCSIC/14744

Dates

Accepted:

2022-09-20

Available:

2022-09-20

Issued:

2022-09

Language

en

Funding References

Funder name: - European Union’s Horizon 2020 research and innovation programme under grant agreement No 730984, ASSEMBLE Plus project. - Spanish MICINN Project SMART (PID2020-112978GB-I00) - The research program LTER-MC is funded by the Stazione Zoologica Anton Dohrn

Rights

openAccess