Data Publication

In-situ Raman spectra from Na2ATP solutions with starting pH 3 and 7 at 80,100 and 120 °C for determination of the hydrolysis rate constant of ATP

Moeller, Christoph | Schmidt, Christian | Guyout, Francois | Wilke, Max

GFZ Data Services

(2024)

Descriptions

Extremophiles maintain an active metabolism up to 122 °C (Takai et al. 2008). These extreme conditions are found, for example in hot springs, in deep oceanic and crustal sediments and in hydrothermal vents at mid-oceanic spreading ridges (Edwards et al., 2011; Heuer et al., 2020). Several studies have investigated the diversity of microorganisms and their relationship to the geological environment as well as to responses to changes. However, the physicochemical parameters necessary to sustain metabolism under these conditions, including the stability of essential molecular compounds like adenosine triphosphate (ATP) and adenosine diphosphate (ADP) have been only studied marginally. Adenosine triphosphate and adenosine diphosphate are essential energy stores in all currently known metabolic systems. In living cells, the energy is released by the enzymatically controlled exergonic hydrolysis of ATP to power other vital endergonic processes. The abiotic hydrolysis of ATP is kinetically enhanced at elevated temperatures and low pH values resulting in a very short lifetime of ATP and ADP in aqueous solutions (Hulett 1970; Khan and Mohan 1974; Leibrock et al. 1995). Therefore, the kinetic stability of ATP plays a crucial role in metabolism at extreme temperatures. This aspect has been proposed as a critical factor in determining the limits of living cells (Bains et al. 2015). This data publication compromises all Raman spectra obtained for solutions of Na2ATP with an initial pH of 3 and 7 at 80 °C, 100 °C and 120 °C and for solutions of Na2ADP with initial pH 5 at 100 °C and 120 °C. A hydrothermal diamond anvil cell (HDAC) coupled to a Raman spectrometer was used for in-situ measurements. Pressure was estimated from the vapor-liquid curve of water. In addition to the Raman spectra, the following data are provided: an assignment of peaks in the fitted spectral range, the initial fit parameters, and the fit results.

Keywords

Originally assigned keywords

ATP

ADP

AMP

Adenosine triphosphate

Adenosine diiphosphate

Adenosine monophosphate

Metabolite

Hydrolysis

Kinetics

In-situ Raman spectroscopy

Hydrothermal diamond anvil cell

High temperature biochemistry

Raman spectra

HDAC

biosphere > biological process > animal life > metabolism > metabolite

chemical process > chemical reaction > reaction kinetics

EARTH SCIENCE > OCEANS > MARINE VOLCANISM > HYDROTHERMAL VENTS

MSL enriched keywords

measured property

pH

Technique

chemical and mineralogical analysis

Raman spectrometry - chemistry and mineralogy

crystal structure analysis

Raman spectrometry - crystal lattice

minerals

chemical elements

carbon

diamond

Apparatus

deformation testing

compression testing

diamond anvil cell

Earth's structure

Earth crust

MSL enriched sub domains i

geochemistry

microscopy and tomography

rock and melt physics

Source

https://dataservices.gfz-potsdam.de/panmetaworks/showshort.php?id=a3e0f5fa-4dbc-11ef-967a-4ffbfe06208e

Source publisher

GFZ Data Services

DOI

10.5880/fidgeo.2024.025

Creators

Moeller, Christoph

Institute of Geosciences, University of Potsdam, Potsdam, Germany

ORCID:

https://orcid.org/0000-0001-7824-8004

Schmidt, Christian

GFZ German Research Centre for Geosciences, Potsdam, Germany

Guyout, Francois

IMPMC Muséum National d'Histoire Naturelle, Paris, France

ORCID:

https://orcid.org/0000-0003-4622-2218

Wilke, Max

Institute of Geosciences, University of Potsdam, Potsdam, Germany

ORCID:

https://orcid.org/0000-0002-1890-3940

Contributors

Moeller, Christoph

DataCollector

Institute of Geosciences, University of Potsdam, Potsdam, Germany

ORCID:

https://orcid.org/0000-0001-7824-8004

Moeller, Christoph

DataCurator

Institute of Geosciences, University of Potsdam, Potsdam, Germany

ORCID:

https://orcid.org/0000-0001-7824-8004

Moeller, Christoph

DataManager

Institute of Geosciences, University of Potsdam, Potsdam, Germany

ORCID:

https://orcid.org/0000-0001-7824-8004

Moeller, Christoph

Researcher

Institute of Geosciences, University of Potsdam, Potsdam, Germany

ORCID:

https://orcid.org/0000-0001-7824-8004

Schmidt, Christian

DataCollector

GFZ German Research Centre for Geosciences, Potsdam, Germany

Schmidt, Christian

Researcher

GFZ German Research Centre for Geosciences, Potsdam, Germany

Guyout, Francois

ProjectMember

IMPMC Muséum National d'Histoire Naturelle, Paris, France

ORCID:

https://orcid.org/0000-0003-4622-2218

Guyout, Francois

Researcher

IMPMC Muséum National d'Histoire Naturelle, Paris, France

ORCID:

https://orcid.org/0000-0003-4622-2218

Wilke, Max

ProjectManager

Institute of Geosciences, University of Potsdam, Potsdam, Germany

ORCID:

https://orcid.org/0000-0002-1890-3940

Wilke, Max

Researcher

Institute of Geosciences, University of Potsdam, Potsdam, Germany

ORCID:

https://orcid.org/0000-0002-1890-3940

Hydrothermal Diamond-Anvil Cell Laboratory

HostingInstitution

GFZ German Research Centre for Geosciences, Potsdam, Germany

Moeller, Christoph

ContactPerson

Institute of Geosciences, University of Potsdam, Potsdam, Germany

References

10.1016/j.bpc.2022.106878

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10.1007/bf00998341

10.1002/jrs.1250190202

10.1038/nrmicro2647

10.1002/jrs.1250190807

10.1126/science.abd7934

10.1038/2251248a0

10.1016/0301-4622(94)00134-6

10.1039/f29898501963

10.1016/0008-6215(84)85398-7

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10.1016/s0006-3495(69)86389-7

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10.1016/j.gca.2008.10.019

10.1180/emu-notes.12.7

10.1073/pnas.0712334105

10.1016/0022-1902(74)80138-7

10.1063/1.1461829

Citation

Moeller, C., Schmidt, C., Guyout, F., & Wilke, M. (2024). In-situ Raman spectra from Na2ATP solutions with starting pH 3 and 7 at 80,100 and 120 °C for determination of the hydrolysis rate constant of ATP [Data set]. GFZ Data Services. https://doi.org/10.5880/FIDGEO.2024.025

Dates

Created:

2024

Issued:

2024

Rights

Creative Commons Attribution 4.0 International